Swarming Media

• 1% (w/v) tryptone
• 0.5% (w/v) yeast extract
• 1% (w/v) NaCl
• 0.6% (w/v) Eiken agar (not regular agar!)
• 0.5% (w/v) Glucose (add from a sterile 20% glucose solution after autoclaving)

Recomendation:
Make 1500mL: 15g tryp, 7.5g yeast, 15g NaCl, 9g agar in total (do not add to batch media)
Partition into 12 bottles with 120mL¹ in each (0.75g agar each)

Swimming Media

• 1% (w/v) tryptone
• 0.5% (w/v) NaCl
• 0.25% (w/v) agar

Reccomendation:
Make 1500 mL: 15g tryptone, 7.5g NaCl
Partition into 120mL bottles with 0.3g agar each

AAS Media

Same thing as swimming media but without the agar
Need approximately 10 grams of cells; from experience a total of 3L has been enough, so make 1L batches in 2L E-flasks x 3.

LB

• mixture in lab; see bottle for preparation instructions