Lab Recipes
Swarming Media 1% (w/v) tryptone 0.5% (w/v) yeast extract 1% (w/v) NaCl 0.6% (w/v) Eiken agar (not regular agar!) 0.5% (w/v) Glucose (add from a sterile 20% glucose solution after …
1 min
Yep Lab
Yep Lab
9 pages
Proposed Assay for CA Supplementation
The following tests the hypothesis that the nonswarming strain fails to swarm under an immobilizing lack of surfactant. Provided this is true, external supplementation of CA should …
2 min
Yep Lab
Colony Counting using YOLOv8n
Model used is YOLOv8n pretrained on colonies1, but optimized for our use. The process is as follows: flowchart TD A["Load image"] --> B["Find Petri dish with …
2 min
Yep Lab
Revision to the Biofilm Assay: The Bladder Cell Invasion Protocol
Since we were having issues with the biofilm formation, as expounded upon in a previous post, Dr. Yep decided to make some revisions to the protocol. This post will be a brief …
3 min
Yep Lab
ASM 2025
This short informal post is long overdue, but I’ll excuse myself since this website wasn’t up and running in June, when I attended the conference—and besides, I’ve been busy with …
3 min
Yep Lab
Issues with Biofilm Formation
In the lab, Kelsey and I have been running into issues with the biofilm assay. Regardless of the incubation period, the E. coli strains that are supposed to form biofilms aren’t …
3 min
Yep Lab
LB, ∆fur, and Stationary Phase
Dr. Yep noticed how ∆fur stalled earlier than the other strains. It’s not necessarily a problem, but I’d like to understand the cause. The paper should also explain this, so we’ll …
3 min
Yep Lab
∆waaF and Colanic Acid as a Surfactant
Over the summer, I got my hands on Escherchia coli ∆waaF. The researchers at UT Austin were kind enough to consider a collaboration. This strain overproduces colanic acid, which I …
3 min
Yep Lab
The Yep Lab: Iron and the Motility of CFT073
PrefaceAfter writing a first draft of this page, which came out to a couple thousand words, I decided to break this post up into multiple posts. In this first one, I will explain …
6 min
Yep Lab