AAS Trial 1 / Standard Operating Procedure
Quantification of Iron in E. coli Cells by Atomic Absorption Spectroscopy PurposeThis protocol describes the use of atomic absorption spectroscopy (AAS) to quantify iron in an E. …
5 min
Lehr Lab
Lehr Lab
3 pages
Calculations for AAS
The reported amount of of iron in WT E. coli is around 10^5 to 10^6 atoms per cell 1. Converting atoms per cell to micrograms per gram of cellsUsing \[ 10^5 \,\text{atoms Fe/cell} …
3 min
Lehr Lab
Iron Quantification using Atomic Absorption Spectroscopy
Since we’re working under the assumption that we have isogenic mutants with varying intracellular iron concentrations, it would only make sense to verify this assumption by …
7 min
Lehr Lab